| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
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| Autores: |
Guevelou, Eric
Huvet, Arnaud
Sussarellu, Rossana
Milan, Massimo
Guo, Xinmei
Li, Li
Zhang, Guofan
Quillien, Virgile
Daniel, Jean-yves
Quere, Claudie
Boudry, Pierre
Corporeau, Charlotte
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| Data: |
2013-07
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| Ano: |
2013
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| Palavras-chave: |
Marine bivalve
Crassostrea gigas
Hypoxia
AMP-activated protein kinase
Alternative splicing
Adductor muscle
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| Resumo: |
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPKα nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPKα and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00119/22998/21397.pdf
DOI:10.1007/s00360-013-0743-6
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| Editor: |
Springer Heidelberg
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| Relação: |
http://archimer.ifremer.fr/doc/00119/22998/
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| Formato: |
application/pdf
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| Fonte: |
Journal Of Comparative Physiology B-biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2013-07 , Vol. 183 , N. 5 , P. 597-611
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| Direitos: |
2013, Springer-Verlag Berlin Heidelberg
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